Radiation Biology

PV QA 1 - Poster Viewing Q&A 1

SU_39_2389 - Enhanced TrkA neurotrophin receptor expression ameliorated irradiation-induced memory decline

Sunday, October 21
1:15 PM - 2:45 PM
Location: Innovation Hub, Exhibit Hall 3

Enhanced TrkA neurotrophin receptor expression ameliorated irradiation-induced memory decline
S. Ji1, Y. Tian2, D. Xin3, D. Tian4, and W. H. Hao4; 1Department of Radiation Oncology, The Affiliated Suzhou Hospital of Nanjing Medical University, suzhou, China, 2Department of Radiotherapy & Oncology, The Second Affiliated Hospital of Soochow University; Institute of Radiotherapy & Oncology, Soochow University; Suzhou Key Laboratory for Radiation Oncology, Suzhou, China, 3Radiotherapy and Oncology, The Affliated Hospital of Xuzhou medical University, xuzhou, China, 4Department of Radiotherapy & Oncology, The Yancheng NO.1 People’s Hospital, Yancheng, China

Purpose/Objective(s): This study aimed to investigate the role of neurotrophin receptor TrkA in radiation-induced memory decline.

Materials/Methods: Single dose of 10Gy 4MV electron beam whole brain irradiation (WBI) was given to young male Sprague-Dawley rats (50–60g, day 21). 3 months after WBI, Western blot was performed to detect protein level of TrkA. The Morris water maze test was performed to evaluate the consequence of WBI on hippocampus-dependent memory formation. For overexpression of TrkA, Adeno-associated virus were injected at the rate of 0.2μl/min (n=15-20 per group). After that, Morris water maze test was performed again.

Results: The results showed that protein level of TrkA expression decreased significantly(32%, p<0.05)at 3 months after irradiation compared with control group. In Morris water maze test, WBI lead to notable memory deficit, the irradiation rats spent longer latency time than control rats (p<0.05). In the spatial probe trial, the irradiation rats spent less time in the target quadrant than control rats (p<0.05). After Adeno-associated virus injection, AAV-overexpression TrkA rats showed less escape latency time (p<0.05) and spent more time (p<0.05) in the target quadrant than AAV-irradiation rats, but still more escape latency time (p<0.05) and spent less time (p<0.05) in the target quadrant than AAV-control rats.

Conclusion: TrkA may play an critical role in radiation-induced memory deficit.

Author Disclosure: S. Ji: None. Y. Tian: None. D. Xin: None. W.H. Hao: None.

Send Email for Shengjun Ji


Assets

SU_39_2389 - Enhanced TrkA neurotrophin receptor expression ameliorated irradiation-induced memory decline



Attendees who have favorited this

Please enter your access key

The asset you are trying to access is locked. Please enter your access key to unlock.

Send Email for Enhanced TrkA neurotrophin receptor expression ameliorated irradiation-induced memory decline