Topical Area: Maternal, Perinatal and Pediatric Nutrition
Objectives : Using a mouse gestational diabetes mellitus (GDM) model, this study investigated whether maternal choline supplementation (MCS) could alter postnatal growth and metabolic abnormalities associated with GDM.
Methods : C57BL/6 mice were either fed a low fat (LF, 10kcal % fat) control diet or a high fat (HF, 60kcal % fat) diet prior to and during pregnancy to induce GDM. These mice received either 25mM choline (MCS) or plain drinking water, After weaning, offspring were fed the HF diet for 6 weeks before glucose tolerance testing and dissection.
Results : In male offspring from MCS-GDM mothers , we observed a decrease in fasting blood glucose levels and an increase in glucose tolerance when comparing to other groups (P < 0.05). Liver choline metabolite measurements demonstrated that free choline content was lower (P = 0.01) in the MCS-GDM male offspring than control GDM male offspring; there is also an increase in liver sphingomyelin concentrations (P = 0.007) in female offspring from MCS-GDM compared to control GDM dams.
Conclusions : MCS during GDM leads to improvements in blood glucose control in male mouse offspring exposed to a postnatal HF environment.
Funding Sources : NIGMS and NIDDK; New York Academy of Sciences