Topical Area: Energy and Macronutrient Metabolism, Obesity
Objectives : The biochemical characteristics of the inhibitory compound to lipase isolated from potato was compared to the commercially available anti-obesity drug, Orlistat.
Methods : Lipase inhibitory compound was isolated from the water extract from potato by serial solvent extraction and chromatographic purifications. The lipase inhibition kinetics was assessed by double reciprocal Lineweaver-Burk plotting. the potency of lipase inhibition of the compound was compared with Orlistat using 4-MUO as a substrate.
Results : The lipase inhibitroy compound exhibited mixed type inhibition and high potency with an estimated IC50 of 216 ng/mL in the impure extract. The lipase inhibition activity of the compound is comparable to the commercially available anti-obesity drug, Orlistat, whose IC 50 was 0.4 ng/mL. The compound is stable at low pH (pH 2) and at boiling temperature for 1 h. Storage stability test showed that the compound is stable at least 4 weeks at RT.
Conclusions : Regarding the stability, solubility in water and lipase inhibition activity, the compound isolated from potato has a potential to be developed as a safe anti-obesity remedy.
Funding Sources : This study was funded by a basic research grant from Korean National Research Fund