Topical Area: Nutritional Microbiology, Nutrient-Gene Interactions
Objectives : Dietary polyphenols promote memory in models of sleep deprivation (SD), stress, and neurodegeneration. The biological properties of dietary polyphenols greatly depend on the bioavailability of their phenolic metabolites derivatives that are modulated by gut microbiota. We recently demonstrated that supplementation with grape-derived bioactive dietary polyphenol preparation (BDPP) improves SD-induced cognitive impairment.
Methods : This study examined the role of gut microbiota in BDPP’s memory promotion effect in SD. C57BL6/J mice, treated with antibiotics or BDPP or both were sleep-deprived at the end of a fear conditioning training session and fear memory was assessed the next day. Gut microbiota composition was analyzed in fecal samples and BDPP-driven phenolic acid metabolites extraction was measured in plasma.
Results : We report that the beneficial effect of BDPP on memory in SD is attenuated by antibiotics-induced dysbiosis. We identified specific communities of fecal microbiota that associate with the bioavailability of BDPP-derived phenolic acids, which in turn, associated with memory promotion.
These results suggest the gut microbiota composition significantly affects the bioavailability of phenolic acids that drive the dietary polyphenols’ cognitive resilience property. Our findings provide a preclinical model to test causal association of gut microbiota-polyphenols in the potential development of dietary polyphenols for the prevention/treatment of cognitive impairment.
Funding Sources : This project was funded a P50 CARBON Center grant from NCCIH/ODS (Pasinetti, PD/PI)