Topical Area: Dietary Bioactive Components
Objectives : Urinary tract infections (UTIs) are the second most common bacterial infections and are often treated with antibiotics. The North American cranberry is recommended for UTI prophylaxis in women with recurrent UTI as a nutritional alternative approach. The ability of cranberry components or their metabolites to inhibit the adhesion of uropathogenic E.coli to the bladder is considered as an important mechanism by which cranberry mitigates UTIs. The objective of this study was to evaluate the anti-adhesion activity of urine from healthy individuals against both type I pili and P-fimbriae uropathogenic E.coli, after the consumption of Cranberry +health™ Cranberry Supplement.
In this randomized, double-blind, placebo-controlled pilot trial (n=20), subjects consumed two cranberry chews, one in the morning and one in the evening. Clean-catch urine samples collected at baseline and post-intervention (0-3h, 3-6h, 6-9h, 9-12h, 12-24h, 24-30h, 30-36h) were tested for anti-adhesion activity utilizing both a mannose-resistant human red blood cell hemagglutination assay specific for P-fimbriae E. coli and a T24 cell line model for type I pili E.coli (UTI89).
Results : Urinary ex vivo anti-adhesion activity against P-fimbriae E. coli after consuming cranberry chew was significantly greater (p < 0.05) than placebo chew at 0-3h, 3-6h, 6-9h, 9-12, and 12-24 time point. Urinary anti-adhesion activity against type I pili E.coli (UTI89) following cranberry chew was significantly greater (p < 0.1) than placebo chew at 3-6h, 6-9h, and 12-24h time point.
Acute consumption of Cranberry +health™ Cranberry Supplement provides ex vivo anti-adhesion activity, which may help to improve urinary tract health.
Funding Sources : Ocean Spray Cranberries, Inc