Topical Area: Global Nutrition
Objectives :
Unreliability of nutritional biomarkers (NB) have been reported in apparently healthy people with subclinical inflammation. The objective of this study was to measure NBs before and after a vaccination induced inflammation. NBs included: Ferritin (Fer), soluble Transferrin Receptor (sTfR), Retinol Binding Protein 4 (RBP4), Thyroglobulin (Tg), 25OH-Vitamin D3 (25OH-D), retinol (VA), Folates, Cobalamins (B12), and inflammatory markers, acid 1- α-Glycoprotein (AGP) and C-Reactive Protein (CRP).
Methods :
Children aged 12-18 months were recruited at ICDDR-B in Dhaka, Bangladesh. Blood samples were drawn initially and 1 day post inoculation with Pentavalent vaccine. Harvested serum was frozen and transported to labs for analysis. Fer, sTfR, RBP4, Tg , AGP, and CRP, were measured in 96-well format by chemiluminescent with the Quansys Human Micronutrient 7 Plex array and the Q-view reader. Serum Folates and Total B12 were estimated microbiologically using ALPCO-Immundiagnostik 96-well kits. Serum 25OH-D and VA were measured by LC-MS/MS.
Results : Vaccine induced inflammation significantly increased CRP, AGP, Fer and sTFR by 4.48mg/L (798% ), 0.435g/L (48%), 9.57µg/L (60%) and 7.6mg/L (31%), respectively. Furthermore, inflammation significantly reduced VA, RBP4, and B12 by 74.7 µg/L (18%), 0.637 mmol/L (34%), and 38.74 ng/L (14%), respectively. Tg, Folates and 25OH-D were not significantly affected by the vaccine induced inflammatory response.
Conclusions : The study confirms that mild inflammation significantly alters the reliability of 7 out of 10 measured nutritional biomarkers after vaccine inoculation. The measurement of inflammatory markers is necessary for accurate determination of nutritional status in young children.
Funding Sources : Funding provided by the Bill and Melinda Gates Foundation
Francisco Arredondo
Eurofins Craft Technologies, Inc
Neal Craft
CEO
Eurofins Craft Technologies, Inc
Matthew Fleshman
Eurofins Craft Technologies
Shaikh Meshbahuddin Ahmad
Associate Scientist
ICDDR,B
Jahangir Alam
ICDDR,B
Nure Afsar
ICDDR,B
Georg Lietz
Human Nutrition Research Centre - Institute of Cellular Medicine, Newcastle University
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