Presentation Authors: Ethan Matz, Winston Salem, NC, Trent VanHorn*, Ryan Terlecki, Winston-Salem, NC, John Jackson, Winston Salem, NC
Introduction: Platelet rich plasma (PRP) is a novel therapeutic increasingly promote for various indications, including several within urology. PRP is a platelet concentrate extracted from whole blood via proprietary separator gel systems. Prevailing theories regarding efficacy suggest that local delivery of trophic factors will promote tissue regeneration. Detailed quantitative analysis of delivered factors is essential and prior investigations have failed to adequately delineate profiles. We sought to characterize PRP cytokine profiles across a spectrum of donors using a single proprietary system.
Methods: Whole blood was collected from 10 healthy volunteers age 20 â€“ 65 using standard technique into 11 or 22cc PRP tubes from Eclipse Aesthetics (The Colony, Texas). Tubes were centrifuged until separated. Concentrated PRP (platelet layer mixed with a minority of the platelet poor layer) was sent immediately for 200 Human Biomarker Testing (Ray Biotec; Norcross, Georgia). Platelet concentration was determined for each sample.
Results: The ten highest factors and their primary roles are demonstrated (Table 1). Across the cohort, relative to whole blood, platelet concentration was 1.91 fold higher in the final product. Platelet concentration in PRP obtained from 22cc tubes was significantly higher than 11cc tubes (p=.037). At p=.05, heat mapping reveals 31 trophic factors responsible for driving variability in expression between 22cc and 11cc samples (Table 2).
Conclusions: The majority of the most concentrated cytokines are growth factors and cell adhesion molecules, correlating with the presumed mechanism of PRP. Collection tube characteristics may affect platelet concentration and the level of trophic factor expression. These findings underscore the difficulty of producing a standardized PRP product and suggest a need for further investigation into isolation of the highly expressed factors and optimization of collection.
Source of Funding: Eclipse Aesthetics