Presentation Authors: Vijay Samineni*, Sienna Sewell, Julian Sakey, St Louis, MO, Robert Gereau IV, St. Louis, MO
Introduction: Interstitial cystitis and bladder pain (IC/BPS) are chronic conditions that affect 8 million women in the United States. IC/BPS is often co-morbid with depression and anxiety. Majority of the patients report that the stress exacerbates bladder pain and elevated levels of IC symptoms, suggesting interaction between stress and IC. To better understand the interaction between the cystitis induced bladder pain and affective state and stress, we used a mouse model of bladder pain and performed longitudinal studies to characterize the timeline of pain sensitization and negative affective behaviors after induction of the bladder pain and the relationship between stress and bladder pain.
Methods: We used chemotherapeutic drug cyclophosphamide (CYP) to induce intense bladder inflammation. Measured mechanical sensitivity using von Frey assay, anxiety using elevated zero maze (EZM) and depression using forced swim test (FST).
Results: We observed CYP induced bladder pain in a dose dependent manner. Mice with a dose of 100mg/kg CYP, exhibited significant referred pain sensitization for 1 week and significant anxiety, depressive and anhedonia behavior for 10 days post. Whereas for a dose of 200mg/kg CYP, significant referred pain sensitization was observed for 6 weeks and significant anxiety, depressive and anhedonia behavior for 2 weeks. CYP induced referred pain sensitization dissipated completely 12 weeks post administration. To determine if stress exposure can reinstate the dissipated bladder pain symptoms in previously CYP treated mice, we exposed these mice to acute stress and tested for referred pain sensitization. Acute stress exposure reinstated referred pain sensitivity in the only in CYP treated mice post extinction of pain. Our data suggests acute stress is a potent inducer for emergence of IC/BPS symptoms.
Conclusions: Our data suggests acute stress is a potent inducer for emergence of IC/BPS symptoms.
Source of Funding: NIDDK K01 Award DK115634 to Vijay Samineni