Category: Organ transplantation
Circular RNAs (circRNAs) that are a new class of endogenously expressed non-coding RNAs produced from back splicing with a covalently closed loop structure have been emerging as an important gene regulator in the physiological and pathological development of cells. It remains unknown about roles of circRNAs in DCs. The objective of the study is to investigate the impact of circular RNA FSCN1 (circFSCN1) on dendritic cell immune function. Bone marrow derived dendritic cells were cultured in vitro and circRNA expression was detected by circRNA microarrays and qRT-PCR. The effect of circFSCN1 on DCs was studied. We found that the expression profiles of circRNAs were significantly different in mature immunogenic DCs v.s immature immunosuppressive DCs. circFSCN1 was the most significantly highly expressed in mature DCs compared with immature DCs. Treatment with immunosuppressive cytokines TGF beta and GDF15 reduced circFSCN1 expression in DCs. Knockdown of circFSCN1 using siRNA reduced the phosphorylation of Rel Ap65, but increased phosphorylated Foxo3. Silencing of circFSCN1 impaired DCs to activate T cells, changed inflammatory cytokine production and enhanced Treg generation whereas circFSCN1 siRNA did not affect DC maturation, suggesting that circFSCN1 siRNA may help induce immunosuppression in organ transplantation. In conclusion, it is a first report to demonstrate that circFSCN1 is essential for DC immune response.