Other - pediatric Systemic Lupus Erythematosus
Patients with Systemic Lupus Erythematosus (SLE) display a complex blood transcriptome whose cellular origin is poorly resolved. Using single-cell RNA-seq, we profiled ~276,000 peripheral blood mononuclear cells (PBMCs) from 33 pediatric SLE, with different disease activity scores, as well as 11 matched healthy controls. Our analysis yielded 20 transcriptionally distinct cell populations, including three monocyte, two B cell, a plasma cell (PC), three CD8+ T cell, and two NK cell clusters. Overall, the SLE signature was comprised of each cell type, although minor populations of PCs and pDCs over contributed. Interferon-stimulated genes (ISGs) were expressed within a restricted SLE CD14+ monocyte population, which correlated with disease activity. While the most prevalent ISGs found in SLE patients were restricted to few clusters, those previously associated with flares spread to every cell type. These results will be compared and validated using an adult SLE cohort and will lay a foundation for resolving the heterogeneity of SLE.