Inflammatory bowel diseases
The immune system protects the body against invaders that cause infection and disease. On the other hand, disorders of the immune system can lead to autoimmunity, inflammatory disease and cancer. The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) protein 9 system (known as CRISPR/Cas9) is a powerful new technology with a wide range of applications in biomedical research, including the potential to treat human genetic disease. To better understand the immune system and discover novel therapeutics for autoimmune diseases, we have built CRISPR/Cas9-based platforms for new target identification, target exploration and validation, and evaluation of the efficacy and toxicity for early targets. In this work, we will describe the methodologies we have established for delivering the CRISPR/Cas9 system to the cell types of interest, and the in vitro and in vivo model systems we have built using CRISPR/Cas9 to study gene function. With CRISPR/Cas9-based platforms, we aim to build a mechanism to continuously screen new targets from various data sources and to explore novel biology involved in the immunological diseases.