Object: Lipopolysaccharide (LPS) has a variety of immune activity and is contained within air and environmental smoke. LPS inhalation also induces neutrophils into the lung and causes lung inflammation in animals. Honey is used as a traditional medicine for colds, skin inflammation but not edible. However, the mechanism of the anti-inflammation activity of honey on lung inflammation are not fully understood. Therefore, we investigated the mechanism of anti-inflammation activity of honey. Materials & Methods: 8-10weeks female C57BL/6 mice were used. Mice were inhaled 600μg of Japanese honey (Kyoto Sangyo University) and following 1 day later, mice were inhaled 60μg of LPS by intranasal administration. Alveolar macrophage (AM) and neutrophil (Neu) were obtained by broncho alveolar lavage (BAL) . The expressions of Gr-1 and hydrogen peroxide production were analyzed by flow cytometry. Chemotactic activity for Neu was measured by EZ-TAXIScan. IL-1β and CXCL1 mRNA expressions of AM were analyzed by RT-PCR. Results: The number of New was significantly (pConclusion: These results suggested that the mechanism of anti-inflammatory effects by honey due to inhibit the infiltration of Neu to lung via suppression of CXCL1 production from AM. Honey also inhibited Neu functions. Honey may be a candidate as anti-inflammatory medicine.