Kee Thai Yeo, MD CM (McGill U, Canada), Diplomate of the American Board of Pediatrics (General Pediatrics, Neonatal-Perinatal Medicine, Pediatric Infectious Diseases)
Translational Immunology Institute/ SingHealth Duke-NUS Academic Medical Centre
We have created a high dimensionality atlas of the healthy human Immunome (EPIC: Extended Poly-dimensional Immunome Characterization) by interrogating the peripheral blood mononuclear cells of over 200 healthy subjects, ranging from cord blood to adult age, with 63 unique mechanistic and phenotypic markers per cell by mass cytometry (CyTOF). EPIC provides a detailed depiction of the architecture of the human healthy Immunome. EPIC analytical and visualization pipeline is based on an open source, web-based R Shiny bioinformatics toolkit. EPIC can be mined in various ways, for instance to follow developmental changes of any given cell subset or to depict the architecture of the Immunome at any given age range. Importantly, we have built and will keep developing datasets from various immune mediated diseases using the same approach. Consequently, by providing the healthy standard, EPIC enables the depiction and dissection of disease-dependent perturbations of the Immunome architecture. EPIC provides a transformational conceptual advance in Translational Immunology from individual subset focused to immune architecture based approach for the understanding of the physiology and pathogenesis of immune mediated mechanisms. We intend to make EPIC available to the entire community in its full capacity.