CD8+ T cells are involved in atopic conditions such as atopic dermatitis, where they have been shown to secrete TH2 cytokines, and asthma. Despite this, the involvement of CD8+ T cells in IgE mediated food allergy is less studied, particularly in humans. We incubated PBMCs from 16 peanut allergic pediatric subjects with or without peanut protein and compared them to another 16 nonallergic children. Based on changes in activation marker expression, we observed a significant (p<0.0001) increase in peanut specific CD8+ T cells in association with peanut allergy. We then incubated PBMCs from 15 HLA-A*02:01+ peanut allergic subjects with a pool of peanut peptides (as opposed to peanut protein in the previous experiment) containing HLA-A*02:01 binding motifs. In 8 of these subjects, the activation of peanut specific CD8+ T cells was observed. Peanut specific CD8+ T cell clones derived from one of these subjects were shown to recognize a single peanut peptide in a sequence dependent manner. In addition, expression of the TH2 chemokine receptor CCR4 was detected in two ways: 1) ex vivo on peanut specific CD8+ T cells from peanut allergic individuals; and 2) on the peanut peptide specific CD8+ T cell clones described above. These results show that peanut specific CD8+ T cells are increased in peanut allergic humans, recognize peanut peptide in a sequence specific manner, and express a homing marker associated with allergic immune responses.