13th Annual Global Embolization Symposium & Technologies
Purpose : The purpose of this investigation is to present the drug-loading capacity, release characteristics, size distribution and surface morphology of bleomycin hydrochloride, which primarily consists of A2 and B2, loaded by Callispheres polyvinyl alcohol embolization microspheres in vitro.
Material and Methods : Quantitative Callispheres（50-150μm, 100-300μm,300-500μm）was obtained and loaded with desired concentrations(5mg/ml, 10mg/ml and 20mg/ml) or dosages(20mg, 40mg, 80mg) bleomycin hydrochloride. Scanning electron microscopy (SEM) used to monitor the morphologies and LS Particle Size Analyzer applied for investigated the diameters before and after drug loading of the microspheres. Drug amount and release subsequently measured by using a validated high-performance and liquid chromatography (HPLC) method over time. Activity after drug release and anti-cancer activity was confirmed in cell culture by using MTT method.
Results : Here, we prepared multi-components bleomycin with loading up to 75mg drug/ml hydrated Callispheres microspheres. As a result, both the loading efficiency and drug eluting was affected by diameters of Callispheres, dosages and concentrations of bleomycin. Moreover, the protein contained release media gave rise to values for the drug-release activities. The Callispheres shown to maintain their size and shape before and after the drug performance testing process. Cytotoxicity assay certified that the maintaining bioactivity of bleomycin and released from the microsphere.
Conclusions : These findings may suggest that engineer multi-component drugs, such as bleomycin loading with Callispheres, could expand the application for TACE.