(P28-019-20) Effect of Marine Phytoplankton (Oceanix) and Undenatured Type II Collagen in Combination with Exercise on Endurance Capacity and Oxidative Stress in Rats

Objectives: To study the effect of exercise training alone and or in combination with  marine phytoplankton (Oceanix, OCX) and undenatured type II collagen (UCII) supplementation on the endurance capacity, pro-inflammatory markers, and antioxidant defense markers in rats.

Methods: A total of 28 male Wistar albino rats were randomly divided into four groups (n = 7) (i) No exercise and no OCX (Control), (ii) Exercise, (iii) Exercise +OCX-I (2.55 mg d/rat) + UC-II (4 mg), iv) Exercise + OCX-2 (5.1 mg d/rat)+UC-II (4 mg). Levels of cholesterol, triglyceride, proinflammatory cytokines (IL-1β, IL-6, TNF-α, COMP, CRP) ,lactate and malondialdehyde (MDA) levels activities of antioxidant enzymes were determined in all the groups.

Results: Run to exhaustion (minutes) improved in the OCX+UC-II treated groups. Levels of cholesterol, triglyceride, proinflammatory cytokines (IL-1β, IL-6, TNF-α, COMP, CRP) decreased by OCX+UC-II supplementation. A significant decrease in lactate and malondialdehyde (MDA) levels and an increase in activities of antioxidant enzymes were observed in the combination of exercise and OCX+UC-II groups.  Exercise+OCX+UC-II treated had lower TNF-α and IL-1β levels in muscle than exercise and control rats (P < 0.001). Muscle sterol regulatory element-binding protein 1c (SREBP-1c), liver X receptors (LXR), ATP citrate lyase (ACLY) and fatty acid synthase (FAS) levels in the exercise + OCX+UC-II group were lower than all groups (P < 0.05). The effectiveness of the high dose of OCX was more pronounced than the low dose of OCX.

Conclusions: These results suggest OCX and UC-II with exercise may enhance lipid metabolism by regulation of gene products involved in lipid and antioxidant metabolism including SREBP-1c, -γ, LXR, ACLY and FAS in rats.

Funding Sources: Lonza