(P15-130-20) Associations of Maternal Non-Nutritive Sweetener Intake During Pregnancy with Childhood BMI Trajectory

Objectives: To examine the extent to which non-nutritive sweetener (NNS; e.g. aspartame) intake during pregnancy is associated with offspring BMI z-score trajectory from birth to 15 years.

Methods: We included 1683 mother-child pairs from Project Viva, a prospective pre-birth-cohort recruited in 1999-2002 in Massachusetts. The main exposure was maternal NNS intake assessed in the 1^st and 2^nd trimesters of pregnancy using a food frequency questionnaire. We defined NNS as servings/day of diet soda + NutraSweet (aspartame packets) averaged across 1^st and 2^nd trimesters. We used linear regression models to examine associations of maternal NSS intake with offspring BMI z-score at each in-person research visit (birth, 6 months, 3 years, 7 years, and 12 years). We also used mixed-effects models to examine associations with BMI z-score trajectory from birth to 15 years, including both research and clinical measures of BMI for 1570 participants with ≥3 BMI z-score values. We adjusted all models for maternal pre-pregnancy BMI, age, race/ethnicity, education, parity, and pregnancy smoking status.

Results: 70% of mothers were white and mean (SD) pre-pregnancy BMI was 24.6 (5.2) kg/m2. Mean (SD; IQR) intake of NNS was 0.23 (0.55; 0.22) servings/day. While maternal NNS intake (per servings/day) was not associated with BMI z-score at birth (β -0.03 units; 95% CI -0.14, 0.08), NNS was associated with higher BMI z-score at 6 months (β 0.17; 0.06, 0.28), 3 years (β 0.13; 0.03, 0.24), 7 years (β 0.16; 0.04, 0.29) and 12 years (β 0.16; 0.01, 0.31). Based on the BMI z-score trajectory, the associations of NNS intake (Q4 vs. Q1) with BMI z-score became stronger with increasing age from 3-14 years (p_interaction with age: < 0.01); e.g. 3 years (β 0.13; 0.02, 0.23), 7 years (β 0.24; 0.11, 0.37) and 12 years (β 0.38; 0.20, 0.57).

Conclusions: Our findings showed higher maternal NNS intake during pregnancy was associated with higher BMI z-score from childhood to early adolescence, and the associations strengthened with increasing age.

Funding Sources: US National Institutes of Health (R01 HD034568, UH3 OD023286)