(P01-096-20) Effects of Gardenia Jasminoides on Serum and Tissue Aging Markers in D-galactose-Induced Aging Rat Model
Objectives: Gardenia Jasminoides can decrease the aging markers and function as an anti-aging substance of D-galactose-induced aging rat model .
Methods: We randomly assigned the 9-week-old rats after 3 weeks of adaptation period into 4 groups(n=8): Normal control diet group(C), D-galactose-induced aging group(A), D-galactose-induced aging with ethanol extracted Tomato group(T) (as a positive control), and D-galactose-induced aging with ethanol extracted Gardenia Jasminoides group(G). All groups except normal control group were injected with D-galactose(300mg/kg) intra-peritoneally (i.p) for 12 weeks, and the C group was injected with 90% saline as a substitute. From the 4th week of galactose injection, the A, T, and G groups were orally administered with either distilled water, ethanol extracted tomato (as a positive control, 20mg/kg) or ethanol extracted Gardenia Jasminoides (20mg/kg) solution. We measured the aging markers of AGEs (Advanced glycation end products), MDA(Malondialdehyde), SOD2(Superoxide Dismutase2) in serum, and MDA(Malondialdehyde) level in liver.
Results: After 12 weeks of treatment, the food intakes and body weight of all groups showed no significant differences. The serum AGEs level significantly increased in A group compare to C group, and the oral administration of ethanol extracted Gardenia Jasminoides significantly decreased the serum AGEs(p<0.05). The D-galactose-induced aging caused the serum MDA concentration significantly elevated, however, Gardenia Jasminoides treatment did not decrease serum MDA significantly. No significant differences in SOD2 of serum and MDA of liver were observed in all groups.
Conclusions: From the results conclusively we suggest that Gardenia Jasminoides may improve aging markers by decreasing AGEs in serum. The liver oxidative such as SOD2, Catalase, and AKT are currently being investigated by western blot.
Funding Sources: No funding
Sookmyung Women`s University Seoul, Seoul-t'ukpyolsi, Republic of Korea
Sookmyung Women's University Seoul, Seoul-t'ukpyolsi, Republic of Korea